上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.
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SF Cell Line 96-well Kit (96 RCT)
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SF Cell Line 96-well Kit (96 RCT)

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产品详情
品牌 LONZA 货号 V4SC-2096
规格 96 RCT 类型 试剂
运输条件 4℃~8℃ 储存条件 4℃~8℃
货期 请咨询

SF Cell Line 96-well NucleofectorTM Kit

Catalog #: V4SC-2096
For medium-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 96-well ShuttleTM Add-On

Product Overview

The SF Cell Line 96-well ShuttleTM Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 96-well ShuttleTM Add-On.

This kit has been determined to be optimal for transfecting following cell types:

  • 293-F (FreeStyle), 32D

  • A-20, A2058, A375, A431, A549, AGS, ARPE-19

  • BetaTC/6, BxPC-3

  • C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1

  • EL4

  • FDC-P1

  • H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C

  • IMR-32

  • J774A.1

  • K-562

  • KG-1

  • L-428, LNCaP

  • MDA-MB-453, MDBK, Molt-4, MV-4-11

  • Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0

  • P19, P815, PC-12, PC-3

  • Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226

  • S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480

  • T2, T84, TF-1

  • U266B1, U-937

  • Vero

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Benefits

  • -High transfection efficiencies in difficult-to-transfect cell lines

  • -Compatible with liquid handling systems for automated high-throughput electroporation

  • -Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


Applications

  • -Transfection of low cell numbers in the range of 5 x 104 to 1 × 106 cells

  • -High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content & Storage

Content

2.25 mL SF Cell Line NucleofectorTM Solution

0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well NucleocuvetteTM Plate (20 μL)