For transfection of mammalian glial cells lacking a cell type specific Optimized Protocol for the NucleofectorTM I/II/2b Device
In addition to various cell type-specific kits for primary glial cells, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian glial cell type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for glial cells. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Content & Storage
Content
2 x 0.45 mL NucleofectorTM Solution for Primary Mammalian Glial Cells
2 x 0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes
