上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.
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SG Cell Line 4D X Kit S (32 RCT)

13800000000
产品详情
品牌 LONZA 货号 V4XC-3032
规格 32 RCT 类型 试剂
运输条件 4℃~8℃ 储存条件 4℃~8℃
货期 请咨询

SG Cell Line 4D-NucleofectorTM X Kit S

Catalog #: V4XC-3032
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-NucleofectorTM X Unit (20 µL format).

Product Overview


The SG Cell Line 4D-NucleofectorTM X Kit S is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • Ba/F3

  • Colo-205

  • H4

  • NIH/3T3

  • Raji, Ramos

  • THP-1

  • WEHI

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Benefits


  • High transfection efficiencies in difficult-to-transfect cell lines

  • Cells maintain functionality post transfection

  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


Applications


  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells

  • Medium throughput applications

  • Optimization of transfection conditions, e.g. substrate titrations


Content & Storage

Content


2 x 0.675 mL SG Cell Line NucleofectorTM Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well NucleocuvetteTM Strips (20 μl)