SE Cell Line 96-well NucleofectorTM Kit
Catalog #: V4SC-1096
For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 96-well ShuttleTM Add-On
Product Overview
The SE Cell Line 96-well ShuttleTM Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 96-well ShuttleTM Add-On.
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38
The respective cell type-specific Optimized Protocols or recommendations are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.
Benefits
-High transfection efficiencies in difficult-to-transfect cell lines
-Compatible with liquid handling systems for automated high-throughput electroporation
-Conditions are transferable between 4D NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
-Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
-High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Content & Storage
Content
2.25 mL SE Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well NucleocuvetteTM Plate (20 μL)
