上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.
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P3 Primary Cell 96-well Kit (96 RCT)

13800000000
产品详情
品牌 LONZA 货号 V4SP-3096
规格 96 RCT 类型 试剂
运输条件 4℃~8℃ 储存条件 4℃~8℃
货期 请咨询

P3 Primary Cell 96-well NucleofectorTM Kit

Catalog #: V4SP-3096
For medium-throughput transfection of primary cells, e.g. human T cells, CD34 or rat neurons, when working with the 96-well ShuttleTM Add-On.

Product Overview


The P3 Primary Cell 96-well ShuttleTM Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 96-well ShuttleTM Add-On.

This kit has been determined to be optimal for transfecting following cell types:

  • B cell (human)

  • CD34+ cell (human)

  • Chondrocyte (human)

  • DC (human)

  • DC (mouse), mature

  • ES (human), H9

  • ES (mouse)

  • Hepatocyte (human)

  • HMEC (human)

  • Keratinocyte (human)

  • Macrophage (human)

  • Monocyte (human)

  • Neuron (rat), brain Hi/Cx

  • NHBE (human)

  • RPE (human)

  • T cell (human), stim.

  • T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.


Benefits


  • High transfection efficiencies for primary cells

  • Compatible with liquid handling systems for automated high-throughput electroporation

  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


Applications


  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells

  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries


Content & Storage

Content


2.25 mL P3 Primary Cell NucleofectorTM Solution

0.5 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 96-well NucleocuvetteTM Plate (20 μl)