SF Cell Line 4D-NucleofectorTM LV Kit XL
Catalog #: V4LC-2020
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
Product Overview
The SF Cell Line 4D-NucleofectorTM LV Kit XL* is suited for larger scale transfection of cell lines in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the LV NucleocuvetteTM Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal NucleofectorTM Solution:
HEK293 suspension clones
CHO-S suspension clones
K-562
U-937
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-NucleofectorTM System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.
Benefits
-Closed Nucleofection of up to 109 cells
-Conditions transferable from existing smaller scale protocols
-Automatic processing via tubing system
-Transfection of up to 20 mL reaction volume in less than 10 minutes
Applications
-For large scale transfection of up to 1 x 109 cells
-Transient production of potential therapeutic proteins or antibodies for construct screening
-Generation of large numbers of transiently modified cell lines for cell-based assays
Content & Storage
Content
22.5 mL SF Cell Line NucleofectorTM Solution
5 mL Supplement 1
1 x LV NucleocuvetteTM Cartridge
2 x 4D-NucleofectorTM LV Reservoir
