上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.

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SF Cell Line 4D-NucleofectorLV Kit L
产品详情
品牌: LONZA 货号: V4LC-2002
规格: KIT 类型: 试剂
运输条件: 4℃~8℃ 储存条件: 4℃~8℃
货期: 请咨询

SF Cell Line 4D-NucleofectorTM LV Kit L

Catalog #: V4LC-2002

For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit

Product Overview

The SF Cell Line 4D-NucleofectorTM LV Kit L* is suited for larger scale transfection of cell lines in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the 1 mL NucleocuvetteTM Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.

This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal NucleofectorTM Solution:

  • HEK293 suspension clones

  • CHO-S suspension clones

  • K-562

  • U-937

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-NucleofectorTM System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.

Benefits

  • -Closed Nucleofection of up to 108 cells

  • -Conditions transferable from existing smaller scale protocols

  • -Simple manual filling via sterile injection port

  • -Transfection with seconds

Applications

  • -For large scale transfection of up to 1 x 108 cells

  • -Transient production of potential therapeutic proteins or antibodies for construct screening

  • -Generation of large numbers of transiently modified cell lines for cell-based assays

Content & Storage

Content

2.25 mL SF Cell Line NucleofectorTM Solution

0.5 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

2 x 1 mL NucleocuvetteTM Cartridge