上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.

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P3 Prim. Cell 4D-Nucleofector LV Kit XL5
产品详情
品牌: LONZA 货号: V4LP-3520
规格: KIT 类型: 试剂
运输条件: 4℃~8℃ 储存条件: 4℃~8℃
货期: 请咨询

P3 Primary Cell 4D-NucleofectorTM LV Kit XL

Catalog #: V4LP-3520
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit

Product Overview

The P3 Primary Cell 4D-NucleofectorTM LV Kit XL* is suited for larger scale transfection of primary cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the LV NucleocuvetteTM Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.

This kit has been determined to be optimal for transfecting following primary cells and many others that use Solution P3 as optimal NucleofectorTM Solution:

  • B cell (human)

  • CD34+ cell (human)

  • DC (human)

  • ES (human), H9

  • Macrophage (human)

  • Monocyte (human)

  • T cell (human), stim.

  • T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

  • -Closed Nucleofection of up to 1 billion primary cells

  • -Conditions transferable from existing smaller scale protocols

  • -Automatic processing via tubing system

  • -Transfection of up to 20 mL reaction volume in less than 10 minutes

Applications

  • -For large scale transfection of up to 1 x 109 cells

  • -Ex-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)

  • -Generation of large numbers of transiently modified primary cells for cell-based assays

Content & Storage

Content

5 x 22.5 mL P3 Primary Cell NucleofectorTM Solution

5 x 5 mL Supplement 1

5 x LV NucleocuvetteTM Cartridge

10 x 4D-NucleofectorTM LV Reservoir