P3 Primary Cell 4D-NucleofectorTM LV Kit XL
Catalog #: V4LP-3520
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
Product Overview
The P3 Primary Cell 4D-NucleofectorTM LV Kit XL* is suited for larger scale transfection of primary cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the LV NucleocuvetteTM Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
This kit has been determined to be optimal for transfecting following primary cells and many others that use Solution P3 as optimal NucleofectorTM Solution:
B cell (human)
CD34+ cell (human)
DC (human)
ES (human), H9
Macrophage (human)
Monocyte (human)
T cell (human), stim.
T cell (mouse), unstim.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Benefits
-Closed Nucleofection of up to 1 billion primary cells
-Conditions transferable from existing smaller scale protocols
-Automatic processing via tubing system
-Transfection of up to 20 mL reaction volume in less than 10 minutes
Applications
-For large scale transfection of up to 1 x 109 cells
-Ex-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)
-Generation of large numbers of transiently modified primary cells for cell-based assays
Content & Storage
Content
5 x 22.5 mL P3 Primary Cell NucleofectorTM Solution
5 x 5 mL Supplement 1
5 x LV NucleocuvetteTM Cartridge
10 x 4D-NucleofectorTM LV Reservoir
