上海锐信生物技术有限公司
Shanghai Ruixin Biotechnology Co., Ltd.
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SE Cell Line 4D X Kit L (24 RCT)

13800000000
产品详情
品牌 LONZA 货号 V4XC-1024
规格 24 RCT 类型 试剂
运输条件 4℃~8℃ 储存条件 4℃~8℃
货期 请咨询

SE Cell Line 4D-NucleofectorTM X Kit L

Catalog #: V4XC-1024
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-NucleofectorTM X Unit (100 µL format).

Product Overview


The SE Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • 3T3-L1, diff., 3T3-L1, undiff.

  • A-10, A7r5,

  • BHK-21, BJ

  • C2C12, CaCo-2, Calu-3, Capan-1, Cos-7

  • DU145

  • GH3

  • HCT116, HeLa, HeLa S3

  • IMR-90

  • Jurkat (DSMZ), Jurkat E6.1

  • KG-1a, L6

  • MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5

  • NCTC clone 929

  • Panc-1

  • T/G-HA VSMC, T-47D

  • U2OS, U-87 MG

  • WI-38

The respective cell type-specific Optimized Protocols are available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.


Benefits


  • High transfection efficiencies in difficult-to-transfect cell lines

  • Cells maintain functionality post transfection

  • Conditions are transferable between 4D-NucleofectorTM System (including 4D-NucleofectorTM including LV Unit), 96-well ShuttleTM System and 384-well NucleofectorTM System


Applications


  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells

  • Low throughput applications


Content & Storage

Content


2.25 mL SE Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel,
24 x Single use pipettes