Product Overview

The SF Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

• 293-F (FreeStyle), 32D

• A-20, A2058, A375, A431, A549, AGS, ARPE-19

• BetaTC/6, BxPC-3

• C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1

• EL4

• FDC-P1

• H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C

• IMR-32

• J774A.1

• K-562

• KG-1

• L-428, LNCaP

• MDA-MB-453, MDBK, Molt-4, MV-4-11

• Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0

• P19, P815, PC-12, PC-3

• Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226

• S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480

• T2, T84, TF-1

• U266B1, U-937

• Vero

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.